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dc.contributor.authorMacNeil, Grant
dc.date.accessioned2019-04-02T12:46:46Z
dc.date.available2019-04-02T12:46:46Z
dc.identifier.urihttp://hdl.handle.net/10222/75411
dc.description.abstractKaposi’s sarcoma-associated herpesvirus (KSHV) is the etiologic agent of the endothelial cell (EC) cancer, Kaposi’s sarcoma, and two B-cell malignancies. In infected cells, the virus exists in two states; the latent program alters cell morphology and inflammatory gene expression, while the lytic cycle produces new virus and promotes tumorigenesis in a paracrine manner. The kaposin locus, located in the latent gene cluster, generates multiple transcripts and gene products in both viral states; however, its function remains unclear. I developed a library of novel recombinant viruses to mutagenize kaposin coding capacity and investigate protein-specific effects during each viral life cycle. I found that while entry and latent gene expression were unaffected by kaposin mutagenesis, the ability to reprogram ECs may be impaired. Furthermore, I identified defects in lytic viral gene expression and virion production in some kaposin-mutant viruses, leading me to suggest new roles for the kaposins in lytic cycle progression.en_US
dc.language.isoenen_US
dc.subjectKSHVen_US
dc.subjectherpesvirusen_US
dc.subjectKaposinen_US
dc.subjectlytic replicationen_US
dc.titleThe complex kaposin locus of Kaposi's sarcoma-associated herpesvirus: defining a new role in the lytic viral replication cycleen_US
dc.typeThesisen_US
dc.date.defence2019-03-14
dc.contributor.departmentDepartment of Microbiology & Immunologyen_US
dc.contributor.degreeMaster of Scienceen_US
dc.contributor.external-examinerDr. Roy Duncanen_US
dc.contributor.graduate-coordinatorDr. Brent Johnstonen_US
dc.contributor.thesis-readerDr. Jan Raineyen_US
dc.contributor.thesis-readerDr. David Langelaanen_US
dc.contributor.thesis-supervisorDr. Jennifer Corcoranen_US
dc.contributor.thesis-supervisorDr. Craig McCormicken_US
dc.contributor.ethics-approvalNot Applicableen_US
dc.contributor.manuscriptsNot Applicableen_US
dc.contributor.copyright-releaseNot Applicableen_US
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