Show simple item record

dc.contributor.authorKshirsagar, Omkar
dc.date.accessioned2019-03-22T15:44:59Z
dc.date.available2019-03-22T15:44:59Z
dc.identifier.urihttp://hdl.handle.net/10222/75366
dc.description.abstractRiboflavin biosynthetic pathway of Sinorhizobium meliloti1021 has a potential alternative enzyme ArfB, essential in riboflavin production for intracellular use. ArfB forms complexes with riboflavin biosynthetic enzymes in S. meliloti1021. The objective of this study was to identify the novel enzymes involved in the riboflavin biosynthesis of riboflavin prototrophic revertant strain Rm1021∆arfBxOa arfB and analyze the free-living phenotypes and the symbiotic phenotypes. Whole genome sequencing was conducted and several SNPs and indels were identified in the genome of revertants, specifically in genes phnM, SMc00185, and argG. The revertant strains could not form effective symbiosis and exhibited lower growth-rate and flavin secretion compare to the control. Occasionally some revertants exhibited effective symbiosis. Secondary revertants were isolated and sequenced for identification of variations. Genetic modifications were observed on the SMb21442 and Oa arfB genes. Characterization of identified genes in future experiments will provide comprehensive understanding of the functions in riboflavin metabolism of α-proteobacteria.en_US
dc.language.isoenen_US
dc.subjectRhizobiumen_US
dc.subjectRiboflavin biosynthesisen_US
dc.subjectNitrogen fixationen_US
dc.titleIDENTIFICATION OF NOVEL COMPONENT OF RIBOFLAVIN BIOSYNTHETIC PATHWAY IN INVASIVE BACTERIAen_US
dc.typeThesisen_US
dc.date.defence2019-03-13
dc.contributor.departmentFaculty of Agricultureen_US
dc.contributor.degreeMaster of Scienceen_US
dc.contributor.external-examinerShawkat Alien_US
dc.contributor.graduate-coordinatorGordon Priceen_US
dc.contributor.thesis-readerGefu Wang-Pruskien_US
dc.contributor.thesis-readerBalakrishnan Prithivirajen_US
dc.contributor.thesis-supervisorSvetlana Yurgelen_US
dc.contributor.ethics-approvalNot Applicableen_US
dc.contributor.manuscriptsNot Applicableen_US
dc.contributor.copyright-releaseNot Applicableen_US
 Find Full text

Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record